RNA

Part:BBa_K4950012:Design

Designed by: Lucas Ng   Group: iGEM23_PennState   (2023-10-12)


BThrom1 Riboswitch Insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Previously published apatamer sequence was required along with its known binding affinity [1]. Design was selected for its high predicted activation ratio and distinctness from other designs.


Source

Designed by De Novo DNA Riboswitch Calculator

References

[1] Liu, X., Zhang, D., Cao, G., Yang, G., Ding, H., Liu, G., Fan, M., Shen, B., & Shao, N. (2003). RNA aptamers specific for bovine thrombin. Journal of molecular recognition : JMR, 16(1), 23–27. https://doi.org/10.1002/jmr.604